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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be observed in many tumors, which leads to chromosome instability, and so may well lead to tumorigenesis. This protein was discovered to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a vital position in selling cell-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have been explained. Two further splice variants had been documented but in-depth nucleotide sequence facts will not be still out there.
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Blocking peptides are peptides that bind especially on the goal antibody and block antibody binding. These peptide commonly contains the epitope recognized from the antibody.
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This guideline outlines additional doable optimization of cellular and immunoassay parameters to guarantee the very best success are...
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This guideline outlines further feasible optimization of cellular and immunoassay parameters to make sure the best possible outcomes are...
Antibodies bound to the blocking peptide not bind towards the epitope around the target protein. By comparing the staining from the blocked antibody vs . the antibody by itself, you can see which staining is distinct.
Goal Details Cyclin E belongs towards the extremely conserved cyclin family, whose users are characterised by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit unique expression and degradation patterns which add to your temporal coordination of every mitotic event. Cyclin E sorts a fancy with and capabilities as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S stage boundary and is particularly degraded as cells development by means of S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by equally antibodies...
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